terms and Conditions
Use of the website
The information contained on this website is for informational purposes only. Although the information is believed to be correct at the time of publication, you must make your own determination as to whether it is suitable for your use. Not all products or services described on this website are available in all jurisdictions or to all potential customers, and nothing contained herein constitutes an offer or solicitation in any jurisdiction or to any potential customer if such Offer or sale not eligible.
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The Site may contain bulletin board services, chat areas, newsgroups, forums, communities, personal pages, calendars and/or other messaging or communication facilities designed to enable you to interact with the general public or groups (collectively, "Community Features") . You agree to use the Community Features only to post, send and receive messages and materials that are appropriate and relevant to the specific Community Features. You agree to use this website only for lawful purposes.
A. Specifically, you agree not to do any of the following while using the Community Features:
1. Defame, abuse, harass, stalk, threaten or violate the legal rights (such as rights of privacy and publicity) of others.
2. Post, post, upload, distribute or disseminate any inappropriate, profane, defamatory, infringing, obscene, indecent or illegal subject, name, material or information.
3. Upload files containing software or other material protected by intellectual property laws (or rights of privacy of publicity), unless you own or control the rights in or have obtained all necessary consents.
4. Upload files that contain viruses, corrupted files, or any other similar software or programs that may harm the operation of another's computer.
5. Intercepting or attempting to intercept email that is not intended for you.
6. Advertise or offer to sell or buy goods or services for any commercial purpose, unless such messages are expressly permitted by such Community Features.
7. Conduct or forward surveys, contests, pyramid schemes or chain letters.
8. Download any file posted by another user of a Community Feature that you know or reasonably should know cannot be legally distributed in such a manner or that you are contractually bound to keep confidential (even though it is available on the Site).
9. Falsify or remove author attributions, legal or other notices or proprietary designations or labeling of the source of software or other material contained in uploaded files.
10. Misrepresenting affiliation with any individual or organization.
11. Engage in any other behavior that restricts or prohibits anyone from using this website, or that the company believes may damage or make the company or website users liable.
12. Violate any applicable law or regulation or violate any code of conduct or other guidelines that may apply to any particular Community Feature.
13. Harvesting or harvesting information about others, including email addresses, without their consent.
B. You understand and acknowledge that you are responsible for any content you submit and that you, not the Company, are solely responsible for such content, including its legality, reliability and appropriateness. If you post for or on behalf of your employer or other entity, you represent and warrant that you have the authority to do so. By uploading or transmitting material to any area of the site, you warrant that the material is yours or is in the public domain or free from ownership or other restrictions and that you have the right to post it on the site. Furthermore, by uploading or transmitting material to any area of the Site, you grant Company a worldwide, irrevocable, royalty-free right to publish, reproduce, use, adapt, edit and/or modify such Materials, all media now known or hereafter discovered worldwide, including on the Internet and the World Wide Web, for advertising, commercial and promotional purposes without further restriction or compensation, except where prohibited by law, and without notice, review or endorsement .
C. The company reserves the right but does not assume any responsibility: (1) Delete any material posted on the website that the company, at its sole discretion, deems inconsistent with the above commitments or is inappropriate for any reason; (2) Terminate any user's access to all or part of the website . However, Company cannot review all material before it is posted on the Site, nor can it guarantee immediate removal of objectionable material after it is posted. Accordingly, Company disclaims all liability for any action or inaction with respect to transmissions, communications, or content provided by third parties. The Company reserves the right to take any action it deems necessary to protect the personal safety of users of the Site and the public; however, the Company disclaims all liability for any person's performance or failure to perform the activities described in this paragraph.
D. Your failure to comply with (A) or (B) above may result in termination of your access to the Site and may subject you to civil and/or criminal liability.
Special Notes on Community Featured Content
Any content and/or opinions uploaded, expressed or submitted through any Community Features or any other public portion of the Site (including password protected areas), and all articles and answers to questions, except those expressly authorized by Company. , are solely the opinions and responsibility of the person or entity making the opinion and do not necessarily reflect the opinion of the company. For example, any recommendation or suggested use of available Company products or services posted through the Community Features does not imply endorsement or recommendation by Company. If you choose to follow any of these recommendations, you do so at your own risk.
Links to Third Party Websites
This website may contain links to other websites on the Internet. The Company is not responsible for the content, products, services, or practices of third-party websites, including, but not limited to, websites linked to or from this Website, websites framed within this Website, or third-party advertisements. Statements about their quality, content or accuracy. The existence of a link from this website to any third-party website does not mean that we approve, endorse or recommend that website. We disclaim all warranties, express or implied, as to the accuracy, legality, reliability or availability of any content on any third-party site. Your use of third-party websites is at your own risk and is subject to the terms and conditions of use of such websites.
You acknowledge and agree that all content on this website (including all information, data, software, graphics, text, images, logos and/or other materials) and their design, selection, compilation, arrangement and assembly are the property of this website Corporation and is protected by United States and international copyright laws. You are authorized to use the Site Content only for personal or legitimate business purposes. You may not copy, modify, create derivative works, publicly display or perform, republish, store, transmit, distribute, delete, remove, augment, add to, participate in the transmission of, license or sell any material on the Web. Websites without Company's prior written consent, but: (a) temporarily store copies of such materials in RAM, (b) store files automatically cached by your web browser for display enhancement, and (c) print A reasonable number of pages are derived from the Site; provided that in each case you do not alter or delete any copyright or other proprietary notices contained in such material. Title or intellectual property rights in any information or materials on this site are not transferred to you, but remain with the Company or the applicable owner of such content.
The company name and logo, and all related names, logos, products and services displayed on the website are trademarks of the company and/or the applicable third party provider. They may not be used or redisplayed without the prior written consent of the company.
The Company disclaims all responsibility for the materials, information and opinions provided on or through this website ("Site Content"). Reliance on the content of the website is at your own risk. Company disclaims all liability for injury or damage resulting from use of any website content.
This site, its content, and the products and services offered on or through this site are provided on an "as is" and "as available" basis, with all faults. Neither Company nor anyone affiliated with Company makes any warranties or representations as to the quality, accuracy or availability of this website. Specifically, but without limitation, the foregoing, neither the Company nor anyone affiliated with the Company warrants or represents that the Site, the Site Content, or the services provided on or through the Site will be accurate, reliable, error-free or uninterrupted; defects will be correct; the website or the server that makes it available are free of viruses or other harmful components; or the website will meet your needs or expectations. COMPANY DISCLAIMS ANY WARRANTIES, EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NON-INFRINGEMENT.
IN NO EVENT WILL COMPANY OR ITS LICENSORS OR CONTRACTORS BE LIABLE FOR DAMAGES OF ANY KIND ARISING OUT OF OR IN CONNECTION WITH YOUR USE OF OR INABILITY TO USE THIS SITE, THE CONTENT ON THIS SITE, ANY SERVICES PROVIDED HEREIN . access to this site or any linked site, including any direct, indirect, incidental, special, consequential or punitive damages, including, but not limited to, delays, service interruptions, viruses, file deletion, or electronic access to the site or site content or services communications, errors, omissions or other inaccuracies, regardless of Company's negligence and whether Company has been advised of the possibility of any such damages, unless prohibited by applicable law.
You agree to indemnify and hold harmless the Company, its officers, directors, agents, employees and others who participate in the Site from any and all liabilities, costs, damages and costs, including reasonable attorneys' fees, arising out of any violation by you use of these terms and conditions of use, your use of the site or any products, services or information obtained from or through the site, your connection to the site, any content you submit to the site through any community features , or your violation of any rights of others.
Governing Law; International Use
These Terms shall be governed by and construed in accordance with the laws of the State of Pennsylvania, without regard to any principles of conflict of laws. You agree that any action at law or in equity arising out of or relating to these Terms and Conditions of Use will be brought exclusively in a state or federal court located in Pennsylvania, and you consent to and submit to the personal jurisdiction of such courts for the purpose of Litigation for such conduct.
These terms and conditions of use apply to users in the United States, Canada and Puerto Rico. If you are accessing this website from outside the United States, Canada or Puerto Rico, please refer to the applicable international website at www.vwr.com for applicable terms and conditions. If you choose to access this website from outside the designated jurisdiction, instead of using the available international website, you agree to these terms and conditions of use and such terms shall be governed by and governed by the laws of the United States and the state. Pennsylvania, we make no representation that the materials or services on this site are appropriate or available for use in other jurisdictions. In all cases, all users are responsible for compliance with local laws.
We respect the intellectual property rights of others and ask our users to do the same. If you believe that your work has been copied and made accessible on the Site in a manner that constitutes copyright infringement, you may notify us by providing our Copyright Agent with the following information:
An electronic or physical signature of a person authorized to act on behalf of the copyright owner;
A description of the copyrighted work that has been infringed in your claim;
the URL or other identification of a specific location on the website where the material that you claim is infringing is located;
your address, phone number and email address;
You represent that you have a good faith belief that the disputed use is not authorized by the copyright owner, its agent, or the law; and
You represent under penalty of perjury that the above information in your notice is accurate and that you are the copyright owner or are authorized to act on behalf of the copyright owner.
You may contact our agents for notification of claimed copyright infringement on the Site at email@example.com.
GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup.What is 5X green GoTaq flexi buffer protocol? ›
The 5X Green GoTaq® Flexi Buffer, contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.What is the function of GoTaq? ›
Green GoTaq® Reaction Buffer is a proprietary buffer containing a compound that increases sample density, and yellow and blue dyes, which function as loading dyes when reaction products are analyzed by agarose gel electrophoresis.How to do hotstart pcr? ›
In practice, a hot-start PCR condition can be attained by adding the polymerase (or any other essential reagent like dNTPs or Mg2+) only when the mixture has reached a temperature higher than Ta and then starting cycles of primer annealing and extension.
The 5X Colorless GoTaq® Flexi Reaction Buffer has the same formulation as the 5X Green GoTaq® Flexi Reaction Buffer but does not contain dyes and is recommended for any applications where absorbance or fluorescence measurements are necessary prior to PCR cleanup. Both buffers are supplied at pH 8.5.What is GoTaq G2 hot start colorless master mix? ›
The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification. Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results.What does a 10X Taq buffer do? ›
Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents. For Research Use Only. Not for use in diagnostic procedures.What is in GoTaq? ›
GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.How can we prevent PCR contamination? ›
- Work in dedicated space. Setup your PCR away from where you analyze PCR results. ...
- Store PCR reagents and PCR products separately. ...
- Aliquot. ...
- Store PCR tubes/tips/racks separately. ...
- Don't flick your tubes open. ...
- Use a master mixer and add your template last. ...
- Train others.
5X GoTaq® Reaction Buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction. The 5X Green GoTaq® Reaction Buffer has two dyes (a blue dye and a yellow dye) that separate during electrophoresis to show migration progress.
Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation. Whereas conventional PCR is often utilized to make exponential copies of your DNA target sequence without an additional temperature-sensitive reaction activation component.Why do we need hot start PCR? ›
Hot Start PCR is a technique that reduces non-specific amplification and offers the convenience of reaction set up at room temperature. The polymerases used in Hot Start PCR are unreactive at ambient temperatures.Why is GC% important for PCR? ›
DNA templates with high GC content (>65%) can affect the efficiency of PCR due to the tendency of these templates to fold into complex secondary structures. This is due to increased hydrogen bonding between guanine and cytosine bases, which can cause the DNA to be resistant to melting.What is hotstart DNA polymerase? ›
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature.What is hotstart polymerase? ›
Hot start PCR is a variant of the polymerase chain reaction (PCR) developed to suppress enzymatic activity (usually Taq DNA polymerase) until the first denaturation step has been accomplished.What is hotstart PCR? ›
Hot start PCR is a modified form of conventional polymerase chain reaction (PCR) that reduces the presence of undesired products and primer dimers due to non-specific DNA amplification at room (or colder) temperatures.What is KAPA2G fast hotstart DNA polymerase? ›
KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.