GoTaq® G2 Flexi DNA Polymerase, Promega (2023)

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What is GoTaq G2 hotstart polymerase? ›

GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup.

What is 5X green GoTaq flexi buffer protocol? ›

The 5X Green GoTaq® Flexi Buffer, contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.

What is the function of GoTaq? ›

Green GoTaq® Reaction Buffer is a proprietary buffer containing a compound that increases sample density, and yellow and blue dyes, which function as loading dyes when reaction products are analyzed by agarose gel electrophoresis.

How to do hotstart pcr? ›

DNA Polymerases

In practice, a hot-start PCR condition can be attained by adding the polymerase (or any other essential reagent like dNTPs or Mg2+) only when the mixture has reached a temperature higher than Ta and then starting cycles of primer annealing and extension.

What is 5X green or colorless GoTaq flexi buffer? ›

The 5X Colorless GoTaq® Flexi Reaction Buffer has the same formulation as the 5X Green GoTaq® Flexi Reaction Buffer but does not contain dyes and is recommended for any applications where absorbance or fluorescence measurements are necessary prior to PCR cleanup. Both buffers are supplied at pH 8.5.

What is GoTaq G2 hot start colorless master mix? ›

The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification. Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results.

What does a 10X Taq buffer do? ›

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents. For Research Use Only. Not for use in diagnostic procedures.

What is in GoTaq? ›

GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

How can we prevent PCR contamination? ›

Avoid Future Contamination
  1. Work in dedicated space. Setup your PCR away from where you analyze PCR results. ...
  2. Store PCR reagents and PCR products separately. ...
  3. Aliquot. ...
  4. Store PCR tubes/tips/racks separately. ...
  5. Don't flick your tubes open. ...
  6. Use a master mixer and add your template last. ...
  7. Train others.
Jan 1, 2015

What is the composition of the GoTaq buffer? ›

5X GoTaq® Reaction Buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction. The 5X Green GoTaq® Reaction Buffer has two dyes (a blue dye and a yellow dye) that separate during electrophoresis to show migration progress.

What is the difference between hot start PCR and regular PCR? ›

Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation. Whereas conventional PCR is often utilized to make exponential copies of your DNA target sequence without an additional temperature-sensitive reaction activation component.

Why do we need hot start PCR? ›

Hot Start PCR is a technique that reduces non-specific amplification and offers the convenience of reaction set up at room temperature. The polymerases used in Hot Start PCR are unreactive at ambient temperatures.

Why is GC% important for PCR? ›

DNA templates with high GC content (>65%) can affect the efficiency of PCR due to the tendency of these templates to fold into complex secondary structures. This is due to increased hydrogen bonding between guanine and cytosine bases, which can cause the DNA to be resistant to melting.

What is hotstart DNA polymerase? ›

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature.

What is hotstart polymerase? ›

Hot start PCR is a variant of the polymerase chain reaction (PCR) developed to suppress enzymatic activity (usually Taq DNA polymerase) until the first denaturation step has been accomplished.

What is hotstart PCR? ›

Hot start PCR is a modified form of conventional polymerase chain reaction (PCR) that reduces the presence of undesired products and primer dimers due to non-specific DNA amplification at room (or colder) temperatures.

What is KAPA2G fast hotstart DNA polymerase? ›

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.


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